Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.
作者： John S. Abrams
DOI: 10.1002/0471142735.IM0620S13
关键词:
摘要: This unit describes a general method for immunoenzymetric assay of mouse and human cytokines. The technique is based on use two anti-cytokine monoclonal antibodies (MAbs), each specific spatially distinct determinant the cytokine. One these coating antibody, other derivatized detecting antibody. A support protocol chemical labeling IgG with NIP hapten group to produce Another producing horseradish peroxidase (HRPO)-conjugated J4, rat IgG1 anti-NIP antibody that confers specificity HRPO detection system used in assay. described this has allowed successful measurement different cytokines wide variety clinical samples including conditioned medium, serum plasma, ascites, amniotic fluid, bronchoalveolar lavage fluid.
sci-hub.se 参考文章(13)
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