Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF.
作者： JEFFREY SCHWARTZ , NILS BILLESTRUP , MARILYN PERRIN , JEAN MARILYN , WYLIE VALE
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摘要: A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 ± 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence eliminated coincubation a 200-fold excess unlabeled CRF. Treatment dexamethasone (10−9−10−7 m) decreased in dose-dependent manner. These results demonstrate utility fluorescent CRF for identification specific sites suggest binding is altered glucocorticoids. (Endocrinology 119: 2376-2382, 1986)
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endojournals.org参考文章(14)
Z. Naor, D. Atlas, R.N. Clayton, D.S. Forman, A. Amsterdam, K.J. Catt, Interaction of fluorescent gonadotropin-releasing hormone with receptors in cultured pituitary cells. Journal of Biological Chemistry. ,vol. 256, pp. 3049- 3052 ,(1981) , 10.1016/S0021-9258(19)69722-X
PETER C. WYNN, JAMES P. HARWOOD, KEVIN J. CATT, GRETI AGUILERA, Regulation of corticotropin-releasing factor (CRF) receptors in the rat pituitary gland: effects of adrenalectomy on CRF receptors and corticotroph responses Endocrinology. ,vol. 116, pp. 1653- 1659 ,(1985) , 10.1210/ENDO-116-4-1653
E. Hazum, P. Cuatrecasas, J. Marian, P. M. Conn, Receptor-mediated internalization of fluorescent gonadotropin-releasing hormone by pituitary gonadotropes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 6692- 6695 ,(1980) , 10.1073/PNAS.77.11.6692
LOUISE M. BILEZIKJIAN, WYLIE W. VALE, Glucocorticoids Inhibit Corticotropin-Releasing Factor-Induced Production of Adenosine 3′,5′-Monophosphate in Cultured Anterior Pituitary Cells* Endocrinology. ,vol. 113, pp. 657- 662 ,(1983) , 10.1210/ENDO-113-2-657
TERRY J. SMITH, RICHARD DANA, ALEXANDER KRICHEVSKY, JOHN P. BILEZIKIAN, MICHAEL SCHONBERG, Inhibition of β-Adrenergic Responsiveness in Muscle Cell Cultures by Dexamethasone Endocrinology. ,vol. 109, pp. 2110- 2116 ,(1981) , 10.1210/ENDO-109-6-2110
MARILYN H. PERRIN, YAIRA HAAS, JEAN E. RIVIER, WYLIE W. VALE, Corticotropin-releasing factor binding to the anterior pituitary receptor is modulated by divalent cations and guanyl nucleotides Endocrinology. ,vol. 118, pp. 1171- 1179 ,(1986) , 10.1210/ENDO-118-3-1171
J Rivier, C Rivier, W Vale, Synthetic competitive antagonists of corticotropin-releasing factor: effect on ACTH secretion in the rat Science. ,vol. 224, pp. 889- 891 ,(1984) , 10.1126/SCIENCE.6326264
P. LEROUX, G. PELLETIER, Radioautographic study of binding and internalization of corticotropin-releasing factor by rat anterior pituitary corticotrophs. Endocrinology. ,vol. 114, pp. 14- 21 ,(1984) , 10.1210/ENDO-114-1-14
K.N. Westlund, P.C. Wynn, S. Chmielowiec, T.J. Collins, G.V. Childs, Characterization of a potent biotin-conjugated CRF analog and the response of anterior pituitary corticotropes Peptides. ,vol. 5, pp. 627- 634 ,(1984) , 10.1016/0196-9781(84)90094-9
V. Giguere, F. Labrie, J. Cote, D. H. Coy, J. Sueiras-Diaz, A. V. Schally, Stimulation of cyclic AMP accumulation and corticotropin release by synthetic ovine corticotropin-releasing factor in rat anterior pituitary cells: site of glucocorticoid action. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 3466- 3469 ,(1982) , 10.1073/PNAS.79.11.3466