Phosphatidylinositol 3-Monophosphate Is Involved in Toxoplasma Apicoplast Biogenesis

摘要: Apicomplexan parasites cause devastating diseases including malaria and toxoplasmosis. They harbour a plastid-like, non-photosynthetic organelle of algal origin, the apicoplast, which fulfils critical functions for parasite survival. Because its essential original metabolic pathways, apicoplast has become target development new anti-apicomplexan drugs. Here we show that lipid phosphatidylinositol 3-monophosphate (PI3P) is involved in biogenesis Toxoplasma gondii. In yeast mammalian cells, PI3P concentrated on early endosomes regulates trafficking endosomal compartments. Imaging T. gondii showed was associated with protein-shuttling vesicles. Interference regular function by over-expression specific binding module led to accumulation vesicles containing peripheral membrane proteins around and, ultimately, loss organelle. Accordingly, inhibition PI3P-synthesising kinase interfered biogenesis. These findings point an unexpected implication this ubiquitous open perspectives how nuclear encoded traffic apicoplast. This study also highlights possibility developing pharmacological inhibitors PI3-kinase as novel