Postmortem redistribution of the heroin metabolites morphine and morphine-3-glucuronide in rabbits over 24 h

摘要: The interpretation of postmortem drug levels is complicated by changes in blood the period, a phenomena known as redistribution. We investigated redistribution heroin metabolites morphine and morphine-3-glucuronide rabbit model. Heroin (1 mg/kg) was injected into anesthetised rabbit; after 1 h, an auricular vein sample taken euthanised. Following death rabbits were placed supine position at room temperature divided three groups namely (1) immediate autopsy, (2) autopsy 30 minutes (3) 24 h death. Various samples which included femoral blood, cardiac lung, liver, kidney, vitreous humour, subcutaneous abdominal fat, bone marrow skeletal muscle taken. analysed with validated LC-MS/MS method. It observed that within there significant increase free concentration compared to antemortem (0.01 ± 0.01 0.05 0.02 mg/L).Various other metabolite concentrations during course experiment various tissues. Principal component analysis used investigate possible correlations between samples. Some but gave poor predictions (>20 % error) when back calculating. results suggest are good model for further studies study understanding required before accurate time possible.