Determination of the Hapten Density of Immuno-Conjugates by Matrix-Assisted UV Laser Desorption/Ionization Mass Spectrometry

摘要: Abstract Information on the hapten densities of immunoconjugates, that is number coupled haptens, important for production antibodies directed towards respective hapten, e.g. a pesticide. Matrix-assisted UV laser desorption mass spectrometry (MALDI) provides sensitive method measurement molecular weight large bioorganic compounds. The resolution approx. 100, currently achievable with this technique in conjunction careful centroiding procedure peaks, sufficient determination density. Different immunoconjugates and labeled enzymes used enzyme immunoassays were investigated order to evaluate density, as inferred from measured differences between conjugated nonconjugated carrier molecule. pesticides methabenzthiazuron, phenoxybenzoic acid derivatives atrazine pyrethroids haptens. Hapten determined spectra compared those based UV...