Microarray-based detection of dye-labeled DNA by SERRS using particles formed by enzymatic silver deposition.
作者： Katharina Konstanze Hering , Robert Möller , Wolfgang Fritzsche , Jürgen Popp
关键词:
摘要: The growing interest in DNA diagnostics is addressed today by microarrays with fluoresence detection. In our approach, we utilize spatially defined arrays of short oligonucleotides on a modified glass surface. Surface enhanced resonance Raman scattering (SERRS) used to obtain molecularly specific spectra the Raman-active dye-labeled DNA. Nanoparticles produced enzymatic silver deposition are as SERS-active substrate. They grow directly and only areas chip. Furthermore, they potentially offer several advantages for SERS nanoparticles characterized their ability use SERS- SERRS-active substrate estimated. Three different dyes investigated potential involvement sequence analysis.
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