Microglial cells in the retina of Carassius auratus: effects of optic nerve crush.

摘要: To study the morphology and distribution of retinal microglial cells goldfish retina in normal conditions after optic nerve crush, we have used nucleoside diphosphatase (NDPase) technique, applied to whole-mounts or sections, for light electron microscopy. In retinas, two populations NDPase-positive were identified: compact associated with vessels on vitreal surface various layers. The had a bipolar multipolar morphology. Bipolar observed fibre layer, visualised ganglion cell layer (GCL), inner plexiform (IPL), outer layer. highest densities IPL where they adopted regular mosaic-like arrangement which occasional spaces occupied by GCL. After an increase number changes NDPase activity, morphology, cells. These showed activity all layers from day 1 15 axotomy, retraction their processes 7. addition, increased GCL between 2 days decreased 4 axotomy. resemble those other regenerating nonregenerating neuronal systems may reflect general response microglia directed help regeneration process.