Human adipose-derived stem cell treatment modulates cellular protection in both in vitro and in vivo traumatic brain injury models

摘要: BACKGROUND Traumatic brain injury (TBI) is a common cause of morbidity and mortality in the civilian population. The purpose this study was to examine effect(s) adipose-derived stem cell (ASC) treatment on cellular functional recovery TBI via both vitro vivo methods. METHODS Cultured neuroblastoma cells, SH-SY5Y, were scratched mimic an model. effect ASC-conditioned medium (CM) death, mitochondrial function, expression inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin 1β [IL-1β], IL-6), as well apoptosis marker FAS, measured. In our model, Sprague-Dawley rats underwent frontal, closed-head Animals randomly received either intravenous human-derived ASCs or saline within 3 hours compared with sham group. Functional evaluated accelerating Rotarod method. On post-TBI Day 3, tissue harvested assessed for damage enzyme-linked immunosorbent assay TNF-α, immunohistochemical staining β-amyloid precursor protein (β-APP). RESULTS Our data show that ASC imparted reduced death (ratio control: 1.21 ± 0.066 vs. 1.01 0.056, p = 0.017), increased viability 0.86 0.009 1.09 0.01, 0.0001), function (percentage 78 6% 68 3%), significantly decreased levels cytokine IL-1β. study, alone, ASC-treated animals showed no difference recovery, lower expressed TNF-α total protein, 0.47 0.01 0.67 0.04; < 0.01), (fluorescence ratio, 0.43 0.05 0.69 0.03; 0.01). CONCLUSIONS Adipose-derived results improved survival, release, evidence neural injury. No seen. These suggest potential aid protection cells following TBI.