Use of in vitro skin recombinants to evaluate cutaneous toxicity: a preliminary study

摘要: AbstractThe cytotoxicity of surfactants (N cetyl-trimethylammonium bromide, sodium dodecyl sulfate, and sorbitan monooleate), formalin, two non-water-soluble formulations was tested using a skin recombinant made human keratinocytes cultured on dead de-epidermized dermis. Histologic observations biochemical parameters (LDH release MTT activity) are closely related. Moreover, as in the vivo situation, cationic surfactant is more toxic than anionic nonionic. Relative formalin markedly reduced reconstructed epidermis comparison with monolayer culture keratinocytes, emphasizing toxicologic importance stratum corneum barrier. show that salicyclic acid, well-known keratolytic agent vivo, induced reduction horny layer thickness recombinant. These preliminary results suggest dermis may prove to be useful vitro model fo...