The matrix form of collagen and basal microporosity influence basal lamina deposition and laminin synthesis/secretion by stratified human keratinocytes in vitro.

摘要: The ability of the collagen matrix form to support formation a basal lamina by cultured normal human epidermal keratinocytes (NHEK) was determined using transmission electron microscopy. forms tested in this study were a) dry type I film and b) gel. NHEK grown for 14 days on following five different substrates: plain plastic culture dishes without addition (PP); overlaid with dry, aldehyde-crosslinked (DCF-P); an gel (GEL-P); Millipore Millicell CM microporous membranes (DCF-CM); (GEL-CM). maintained 2 wk PP DCF-P unable secrete lamina. GEL-P GEL-CM substrates, however, secreted contiguous at GEL-NHEK interface. To determine if appearance correlated laminin synthesis, immunoprecipitated from cellular extracts, as well media apical chambers. substrate synthesized more than did other four alternative substrates. In addition, able direct compartment DCF-CM Taken together, data indicate that can influence deposition, trafficking NHEK.