Identification of Genes Potentially Regulated by Human Polynucleotide Phosphorylase (hPNPaseold-35) Using Melanoma as a Model

摘要: Human Polynucleotide Phosphorylase (hPNPaseold-35 or PNPT1) is an evolutionarily conserved 3′→5′ exoribonuclease implicated in the regulation of numerous physiological processes including maintenance mitochondrial homeostasis, mtRNA import and aging-associated inflammation. From RNase perspective, little known about RNA miRNA species it targets for degradation whose expression regulates; except c-myc miR-221. To further elucidate functional implications hPNPaseold-35 cellular physiology, we knocked-down overexpressed human melanoma cells performed gene analyses to identify differentially expressed transcripts. Ingenuity Pathway Analysis indicated that knockdown resulted significant changes associated with dysfunction cholesterol biosynthesis; whereas overexpression caused global cell-cycle related functions. Additionally, comparative between our datasets allowed us 77 potential “direct” 61 “indirect” which formed correlated networks enriched wound healing association, respectively. These results provide a comprehensive database genes responsive levels; along identification new candidate offering fresh insight into pathways regulated by PNPT1 may be used future possible therapeutic intervention mitochondrial- inflammation-associated disease phenotypes.