Voltage dependent gating and single channel analysis of heterotypic gap junction channels formed of Cx45 and Cx43

摘要: Human Cx45 and Cx43 gap junction proteins expressed in single mammalian cells form functional channels when paired with themselves one another. In heterotypic pairs, voltage dependence was strongly rectifying, unitary conductances were intermediate between those of homotypic channels, Lucifer Yellow permeability absent. These properties are consistent predicted for pairing hemichannels the observed junctions, where both types close response to negative voltages.