Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors

摘要: Fungi occupy an important ecological niche in the marine environment, and fungi possess immense biotechnological potential. This study documents fungal diversity associated with 39 species of sponges determines their potential to produce secondary metabolites capable interacting mammalian G-protein-coupled receptors involved blood pressure regulation. Total genomic DNA was extracted from 563 representative strains obtained collected by SCUBA Caribbean Pacific regions Panama. A total 194 operational taxonomic units were found 58 % represented singletons based on internal transcribed spacer (ITS) partial large subunit (LSU) rDNA regions. Marine highly dominated Ascomycota (95.6 %) two major classes, Sordariomycetes Dothideomycetes. Rarefaction curves showed no saturation, indicating that further efforts are needed reveal entire at this site. Several unique clades during phylogenetic analysis highest order Pleosporales. From 65 cultures tested determine vitro effect angiotensin endothelin receptors, extracts Fusarium sp. Phoma blocked activation these more than 50 control seven others inhibited between 30 45 %. Our results indicate Panama a “hot spot” as well rich resource for capturing, cataloguing, assessing pharmacological substances present previously undiscovered sponges.