Preparation of Nafion/polycation layer-by-layer films for adsorption and release of insulin
作者： Kentaro Yoshida , Katsuhiko Sato , Tetsuya Ono , Takenori Dairaku , Yoshitomo Kashiwagi
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摘要: Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL by immersion in an insulin solution. The characterized a quartz crystal microbalance atomic force microscope. release from UV-visible adsorption spectroscopy fluorescence emission spectroscopy. greatest amount NAF-PAH film. (NAF/PAH)₅NAF 1 mg mL-1 solution at pH 7.4 61.8 µg cm-2. released higher when immersed solutions 2.0 9.0 than 7.4. Therefore, NAF-polycations could be employed delivery under mild conditions control system according to change.
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