Interactions of release factor RF3 with the translation machinery.

摘要: The bacterial release factor RF3 is a GTPase that has been implicated in multiple, incompletely understood steps of protein synthesis. This study explores the genetic interaction with other components translation machinery. contributes to termination by recycling class I factors RF1 and RF2 off post-termination ribosomes. also dissociation peptidyl-tRNAs from elongating ribosomes post-peptidyltransferase quality control (post-PT QC) mechanism selectively terminates carrying erroneous peptides. A majority vivo studies on have carried out K-12 strains Escherichia coli which carry partially defective an Ala Thr substitution at position 246. Here, contribution specific variant activities investigated. Strain reconstruction experiments both E. Salmonella enterica demonstrate defects post-PT QC are associated loss, as well phenotypes uncovered phenotypic profiling, all substantially ameliorated when active K-12-specific replaced fully Ala246 RF2. These results indicate loss tolerated bacteria factors, but many previously reported for deletion compromised presence