Alpha 3, alpha 5, and beta 4: three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster.

摘要: We have identified two additional members of the neuronal nicotinic acetylcholine receptor (nAChR)-related gene family. cDNA clones for one new gene, designated alpha 5, were isolated from rat hippocampus and PC12 cell line libraries. The 5 encodes a protein 48,800 daltons (424 amino acids) which exhibits significant overall acid sequence identity with previously cloned nAChR subunits 1 (49%), 2 (55%), 3 (52%), 4 (49%). Features characteristic other alpha-subunits are present such as conserved cysteine residues at positions 127, 141, 191, 192, four strongly hydrophobic domains. A second addition to nAChR-related family, beta 4, is encoded in overlapping genomic lambda DD15 RG518A. encoding mature 53,300 (475 acids), consists 6 exons has transcription unit length approximately 18 kilobase pairs. shows considerable (43%), (64%), (44%) subunits. Northern blots showed that, along 2, transcripts both genes line, while situ hybridization experiments demonstrated expression small number nuclei central nervous system. Finally, that encode 3, proteins transcribed convergent polarities form tightly linked cluster spanning 60