Genotypic characterization of Shiga toxin-producing Escherichia coli O157:H7 isolates in food products from china between 2005 and 2010
作者： Li Bai , Yunchang Guo , Ruiting Lan , Yinping Dong , Wei Wang
DOI: 10.1016/J.FOODCONT.2014.08.045
关键词:
摘要: Abstract Shiga toxin-producing Escherichia coli (STEC) O157 is an important foodborne pathogen. The aims of this study were to determine genetic relatedness STEC isolated from foods in China. isolates food characterized by virulence gene typing, antibiotyping, Multi-locus sequence typing (MLST), pulsed-field gel electrophoresis analysis (PFGE), variable number tandem repeat (MLVA), and the single nucleotide polymorphism (SNP) clade typing. Of 30 analyzed, all harbored eae, exhA, stx1 and/or stx2 genes with stx2c subtype predominating. By MLST, they relatively homogenous only 4 STs. PFGE MLVA generated 22 pulsotyples 23 patterns, respectively, which showed considerable diversity. Only one 8 isolate was detected. These results indicate that China heterogeneous. There no correlation between genotypic characteristics sources isolates. Since different subtyping methods often give discriminatory powers, use more than approach necessary providing a accurate picture diversity O157. Four ready-to-eat meat or salads, underscores risk infections. need for surveillance clinically implementation prevention strategies prevent outbreaks infections
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