In vitro cultivation and characterization of Leishmania chagasi amastigote-like forms.
作者： Ashwini Somanna , Vasanthakrishna Mundodi , Lashitew Gedamu
DOI: 10.1016/S0001-706X(02)00054-2
关键词:
摘要: For the first time, we have reported establishment and serial propagation of an axenic culture Leishmania chagasi amastigote-like forms. Parasites were characterized by microscopic evaluation expression two stage-specific genes, A2 Ldccys2 amastigote-specific cysteine protease. The differentiated forms maintained cultivation.
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sci-hub.se 参考文章(17)
K.-P. Chang, Carol Ann Nacy, Richard D. Pearson, [43] Intracellular parasitism of macrophages in Leishmaniasis: In Vitro systems and their applications Methods in Enzymology. ,vol. 132, pp. 603- 626 ,(1986) , 10.1016/S0076-6879(86)32045-7
Archangel Levi Omara-Opyene, Lashitew Gedamu, Molecular cloning, characterization and overexpression of two distinct cysteine protease cDNAs from Leishmania donovani chagasi Molecular and Biochemical Parasitology. ,vol. 90, pp. 247- 267 ,(1997) , 10.1016/S0166-6851(97)00158-8
Genping Ke, Jacques Mauël, Denis Rivier, Leishmania mexicana: extracellular proton concentration is a key regulator of cysteine proteinase CPb expression. Experimental Parasitology. ,vol. 90, pp. 58- 64 ,(1998) , 10.1006/EXPR.1998.4310
Theresa A. Glaser, Steven J. Wells, Terry W. Spithill, John M. Pettitt, Danielle C. Humphris, Antony J. Mukkada, Leishmania major and L. donovani: a method for rapid purification of amastigotes. Experimental Parasitology. ,vol. 71, pp. 343- 345 ,(1990) , 10.1016/0014-4894(90)90039-F
Patricia S Doyle, Juan C Engel, Paulo FP Pimenta, Pedro Pinto da Silva, Dennis M Dwyer, Leishmania donovani: Long-term culture of axenic amastigotes at 37 °C Experimental Parasitology. ,vol. 73, pp. 326- 334 ,(1991) , 10.1016/0014-4894(91)90104-5
Nidhi Gupta, Neena Goyal, Anil K. Rastogi, In vitro cultivation and characterization of axenic amastigotes of Leishmania Trends in Parasitology. ,vol. 17, pp. 150- 153 ,(2001) , 10.1016/S1471-4922(00)01811-0
Y Saar, CHARACTERIZATION OF DEVELOPMENTALLY-REGULATED ACTIVITIES IN AXENIC AMASTIGOTES OF LEISHMANIA DONOVANI Molecular and Biochemical Parasitology. ,vol. 95, pp. 9- 20 ,(1998) , 10.1016/S0166-6851(98)00062-0
W L Roberts, P M Rainey, Antileishmanial activity of sodium stibogluconate fractions. Antimicrobial Agents and Chemotherapy. ,vol. 37, pp. 1842- 1846 ,(1993) , 10.1128/AAC.37.9.1842
SIMONE EPERON, DIANE McMAHON-PRATT, I. Extracellular Cultivation and Morphological Characterization of Amastigote‐Like Forms of Leishmania panamensis and L. braziliensis Journal of Eukaryotic Microbiology. ,vol. 36, pp. 502- 510 ,(1989) , 10.1111/J.1550-7408.1989.TB01086.X
F Faraut-Gambarelli, R Piarroux, M Deniau, B Giusiano, P Marty, G Michel, B Faugère, H Dumon, In vitro and in vivo resistance of Leishmania infantum to meglumine antimoniate: a study of 37 strains collected from patients with visceral leishmaniasis. Antimicrobial Agents and Chemotherapy. ,vol. 41, pp. 827- 830 ,(1997) , 10.1128/AAC.41.4.827