Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)
作者： Claudia Cerella , Christiane Scherer , Silvia Cristofanon , Estelle Henry , Awais Anwar
DOI: 10.1007/S10495-009-0328-8
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摘要: Naturally occurring organic sulfur compounds (OSCs), such as linear allylsulfides from Allium species, are attracting attention in cancer research, since several OSCs were shown to act beneficially both chemoprevention and chemotherapy, while hardly exerting any harmful side effects. Hence, we investigated the possible role of different treatment leukemia. Thereby, found that tested this study induced apoptosis U937 cells, with an efficiency depending on number sulfides, selected most promising candidate, diallyltetrasulfide (Al2S4), for detailed mechanistic studies. Here show Al2S4 accumulation cells early mitosis (G2/M phase), followed by activation caspase-dependent apoptosis. The compound counteracted anti-apoptotic Bcl-2 family members (Bcl-xL, phospho-Bad Bcl-2), promoted Bax Bak release cytochrome c into cytoplasm. Treatment let identification apoptotic events including Bcl-xL degradation, late proteolysis, activation, Bad dephosphorylation, caspase nuclear fragmentation phosphatidylserine exposure.
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J.W. Gray, P. Coffino, [19] Cell cycle analysis by flow cytometry Methods in Enzymology. ,vol. 58, pp. 233- 248 ,(1979) , 10.1016/S0076-6879(79)58140-3
L. Ghibelli, C. Fanelli, G. Rotilio, E. Lafavia, S. Coppola, C. Colussi, P. Civitareale, M. R. Ciriolo, Rescue of cells from apoptosis by inhibition of active GSH extrusion The FASEB Journal. ,vol. 12, pp. 479- 486 ,(1998) , 10.1096/FASEBJ.12.6.479
Philippe Bouillet, Andreas Strasser, BH3-only proteins - evolutionarily conserved proapoptotic Bcl-2 family members essential for initiating programmed cell death. Journal of Cell Science. ,vol. 115, pp. 1567- 1574 ,(2002) , 10.1242/JCS.115.8.1567
Verena M. Dirsch, Alexander L. Gerbes, Angelika M. Vollmar, Ajoene, a Compound of Garlic, Induces Apoptosis in Human Promyeloleukemic Cells, Accompanied by Generation of Reactive Oxygen Species and Activation of Nuclear Factor κB Molecular Pharmacology. ,vol. 53, pp. 402- 407 ,(1998) , 10.1124/MOL.53.3.402
X WU, F KASSIE, V MERSCHSUNDERMANN, Induction of apoptosis in tumor cells by naturally occurring sulfur-containing compounds. Mutation Research-reviews in Mutation Research. ,vol. 589, pp. 81- 102 ,(2005) , 10.1016/J.MRREV.2004.11.001
Claus Jacob, Awais Anwar, THE CHEMISTRY BEHIND REDOX REGULATION WITH A FOCUS ON SULPHUR REDOX SYSTEMS Physiologia Plantarum. ,vol. 133, pp. 469- 480 ,(2008) , 10.1111/J.1399-3054.2008.01080.X
Lindsey A. Allan, Nick Morrice, Suzanne Brady, Gareth Magee, Shalini Pathak, Paul R. Clarke, Inhibition of caspase-9 through phosphorylation at Thr 125 by ERK MAPK Nature Cell Biology. ,vol. 5, pp. 647- 654 ,(2003) , 10.1038/NCB1005
S. Cristofanon, S. Nuccitelli, M. D’Alessio, M. Dicato, M. Diederich, L. Ghibelli, Oxidation-dependent maturation and survival of explanted blood monocytes via Bcl-2 up-regulation. Biochemical Pharmacology. ,vol. 76, pp. 1533- 1543 ,(2008) , 10.1016/J.BCP.2008.07.042
Dong Xiao, Sunga Choi, Daniel E Johnson, Victor G Vogel, Candace S Johnson, Donald L Trump, Yong J Lee, Shivendra V Singh, Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-Jun N-terminal kinase and extracellular-signal regulated kinase-mediated phosphorylation of Bcl-2. Oncogene. ,vol. 23, pp. 5594- 5606 ,(2004) , 10.1038/SJ.ONC.1207747
Eric Block, The Organosulfur Chemistry of the GenusAllium - Implications for the Organic Chemistry of Sulfur Angewandte Chemie International Edition in English. ,vol. 31, pp. 1135- 1178 ,(1992) , 10.1002/ANIE.199211351