Human fibulin-4: analysis of its biosynthetic processing and mRNA expression in normal and tumour tissues.

摘要: Abstract Here, we report the identification of a human orthologue fibulin-4, along with analysis its biosynthetic processing and mRNA expression levels in normal tumour tissues. Comparative sequence fibulin-4 cDNAs revealed apparent polymorphisms signal that could account for previously reported inefficient secretion transfectants. In vitro translation presence full-length truncated polypeptides, latter apparently generated from an alternative initiation site. Since this polypeptide failed to incorporate into endoplasmic reticulum membrane preparations, it was concluded lacked thus represent intracellular form fibulin-4. Using fluorescence situ hybridisation analysis, gene localised chromosome 11q13, region being syntenic portions mouse chromosomes 7 19. Considering fact translocations, amplifications other rearrangements 11q13 are associated variety cancers, evaluated series colon tumours. Reverse transcription-polymerase chain reaction RNA paired adjacent tissue biopsies showed significant proportion tumours had ∼2–7-fold increases level expression. Taken together, results here suggest protein may exist dysregulated is tumourigenesis.