Effect of Oxidation of Glutathione and Membrane Thiol Groups on Mitochondrial Functions

摘要: Diamide [diazenedicarboxylic acid bis (N,N’-dimethylamide)] a thiol oxidant, does not titrate more than 25% of total liver mitochondrial groups. These include the nonprotein thiols present in inter-membrane and matrix spaces vicinal pairs protein membrane-bound -SH. Mitochondrial glutathione content is decreased by diamide only at concentrations above 0.5 mil. At below those required for GSH oxidation, slightly stimulates ATPase activity intact rat mitochondria induces an efflux endogenous Mg2+ dependent on coupled respiration. effects are fully preventy EGTA ruthenium red, known inhibitor Ca2+ transport across inner membrane. The dependence these phenomena energy-dissipating flux also supported release state 4 In presence low ( mM) initially taken up but subsequently released. Concomitantly Mg2+, respiration sensitive to becomes “passive” insensitive red coincidence with release. It assumed that redox some -SH (those concentrations) might control, through modulation flux, binding membranes as well their permeability properties.