Partial characterization and isolation of 130 kDa antigenic protein of Dicrocoelium dendriticum adults.

摘要: The study focused on characterizing and isolating Dicrocoelium dendriticum antigens or their fractions that could be used for the immunological diagnosis of dicrocoeliosis. Somatic (SoAg) excretory-secretory (ESAg) were analyzed by SDS-PAGE specificity was evaluated Western blot with homologous heterologous sera. partially purified chromatographic techniques gel-filtration (Sephacryl S-300) ion exchange (Hitrap-DEAE-Sepharose). analysis using sera ovine infected D. revealed eight main antigenic polypeptides ranging from 24 to 205 kDa SoAg seven ESAg apparent molecular mass in range 26-205 kDa. We detected a specific parasite protein an approximate weight 130 gels, arranged as 450 tetramer native conditions. It also showed strong immunoreactivity against experimentally dendriticum. Gel filtration chromatography other proteins, one about another 42 ESAg. elution conditions (130 monomer) DEAE similar those somatic antigen (pH 7.2, 0.1M NaCl, 29-34 ml fractions) excretion-secretion 8.0, 29-35 fractions). suitability polypeptide infection confirmed pool well individual serum samples sheep. sequence amino termini both same identical reported peptide described globin. This appreciable similarity end globins some phylogenetically related worms.