Dynamics of African swine fever virus shedding and excretion in domestic pigs infected by intramuscular inoculation and contact transmission

摘要: African swine fever virus (ASFV) is a highly virulent pathogen that has spread across Eastern Europe since 2007 and for which there no effective vaccine or treatment available. The dynamics of shedding excretion not well known this currently circulating ASFV strain. Therefore, susceptible pigs were exposed to intramuscularly infected with the Georgia 2007/1 strain measure those through within- between-pen transmission scenarios. Blood, oral, nasal rectal fluid samples tested presence by titration (VT) quantitative real-time polymerase chain reaction (qPCR). Serum was ASFV-specific antibodies. Both intramuscular inoculation contact resulted in development acute disease all although experiments indicated pathogenesis might be different, depending on route infection. Infectious first isolated blood among inoculated day 3, then chronologically direct indirect pigs, 10 13, respectively. Close onset clinical signs, higher titres found compared pigs. No infectious oral genome copies detected. Only one animal developed antibodies starting after 12 days post-inoculation. results provide data domestic suggest limited potential isolate cause persistent