MicroRNA signatures differentiate Crohn’s disease from ulcerative colitis

摘要: Excessive and inappropriate immune responses are the hallmark of several autoimmune disorders, including inflammatory bowel diseases (IBD): Crohn’s disease (CD) ulcerative colitis (UC). A complex etiology involving both environmental genetic factors influences IBD pathogenesis. The role microRNAs (miRNAs), noncoding RNAs involved in regulating numerous biological processes, to pathology, terms initiation progression, remains ill-defined. In present study, we evaluated relationship between colon, peripheral blood, saliva whole miRNome expression patients non-inflammatory (non-IBD) controls identify miRNAs that could discriminate CD from UC. Quantitative real-time PCR (qRT-PCR) was used validate assess miRNA expression. Microarray analysis demonstrated upwards twenty six were changed UC colon biopsies relative non-IBD controls. associated with differential 10 while 6 matched tissues. altered 9 blood. Expression miR-101 miR-21, miR-31, miR-142-3p, miR-142-5p saliva. Our results suggest there is specific patterns versus three separate tissue/body fluids (colon, saliva). Further, aberrant profiles indicate may be contributory pathogenesis, or at least reflect underlying inflammation. Scrutinizing blood samples beneficial monitoring diagnosing patients. panel (miR-19a, miR-101, miR-146a, miR-375) as markers