作者: Philippe Herman-Bausier , Cécile Formosa-Dague , Cécile Feuillie , Claire Valotteau , Yves F. Dufrêne
DOI: 10.1016/J.JSB.2015.12.009
关键词:
摘要: Staphylococcus epidermidis and aureus are two important nosocomial pathogens that form biofilms on indwelling medical devices. Biofilm infections difficult to fight as cells within the biofilm show increased resistance antibiotics. Our understanding of molecular interactions driving bacterial adhesion, first stage formation, has long been hampered by paucity appropriate force-measuring techniques. In this minireview, we discuss how atomic force microscopy techniques have enabled shed light forces at play during staphylococcal adhesion. Specific highlights include study binding mechanisms adhesion molecules means single-molecule spectroscopy, measurement involved in whole cell using single-cell probing nanobiophysical properties living bacteria via multiparametric imaging. Collectively, these findings emphasize notion function tightly connected