Near-Real-Time Analysis of the Phenotypic Responses of Escherichia coli to 1-Butanol Exposure Using Raman Spectroscopy

摘要: Raman spectroscopy was used to study the time course of phenotypic responses Escherichia coli (DH5α) 1-butanol exposure (1.2% [vol/vol]). is interest for bacterial phenotyping because it can be performed (i) in near real time, (ii) with minimal sample preparation (label-free), and (iii) spectral interference from water. Traditional off-line analytical methodologies were applied both 1-butanol-treated control cells draw correlations data. Here, distinct sets bands are presented that characterize traits E. maximized correlation measurements. In addition, observed 1.2% (vol/vol) included following: decreased saturated fatty acids levels, retention unsaturated low levels cyclopropane acids, increased membrane fluidity following initial response rigidity, (iv) no changes total protein content or protein-derived amino acid composition. For most traits, coefficients between traditional approaches exceeded 0.75, major trends captured. The results suggest near-real-time suitable approximating metabolic physiological subjected toxic environmental conditions.