Fibre-type specificity of interleukin-6 gene transcription during muscle contraction in rat: association with calcineurin activity.
作者： Sébastien Banzet , Nathalie Koulmann , Nadine Simler , Olivier Birot , Hervé Sanchez
DOI: 10.1113/JPHYSIOL.2005.089193
关键词:
摘要: In this study, we quantified the transcription of interleukin-6 (IL-6) gene in individual fibres and associated changes calcineurin activity assessed at cellular level during prolonged muscle contraction. Individual myofibres were isolated from plantaris soleus muscles rats end an exhaustive running exercise test (n = 10), categorized according to their myosin heavy chain isoform content, compared those resting 10). Using real-time PCR analysis fibres, a marked rise IL-6 transcript levels occurred type I IIa (P < 0.05). Transcription encoding for modulatory calcineurin-interacting protein-1 (MCIP-1), sensitive indicator activity, also mainly increased Moreover, slight increase MCIP-1 mRNA was observed IIx Fibre types determined by immunohistochemistry qualitatively examined glycogen content using periodic acid-Shiff staining, no direct relationship found, level, between fibre-type transcription. Our data clearly suggest that early recruited contraction-induced could be with enhanced activity.
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