Within Germinal Centers, Isotype Switching of Immunoglobulin Genes Occurs after the Onset of Somatic Mutation
作者： Edward E Max , Héctor Martinez-Valdez , Yong Jun Liu , Florence Malisan , Odette de Bouteiller
DOI: 10.1016/S1074-7613(00)80432-X
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摘要: Abstract Human tonsillar B cells were separated into naive IgD + CD38 − CD23 (Bm1) and (Bm2), germinal center centroblasts (Bm3) CD77 centrocytes (Bm4), memory (Bm5) subsets. Previous IgV H sequence analysis concluded that the triggering of somatic mutations occurs during transition from Bm2 subset Bm3 subset. To determine initiation isotype switching, sterile transcript expression was analyzed by amplification, cloning, sequencing. A selective Iγ, Iα, Ie observed at centrocyte (Bm4) stage, suggesting switch is triggered within centers, after mutation initiated (Bm3). Finally, high level 5′Sγ–Sμ3′ DNA switching circles in indicates human tonsils, a major location for switching.
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