Side effects of control treatment can conceal experimental data when studying stress responses to injection and psychological stress in mice.

摘要: Routine laboratory procedures, such as handling or transporting animals carrying out injections on animals, are stressful for but necessary in many pre-clinical studies. Here, the authors show that multiple of non-toxic vehicle cyclodextrin moderately increased plasma corticosterone concentrations female BALB/c mice. Additionally, male mice had received a single intraperitoneal injection harmless saline an glucocorticoid response to second injection. The found been exposed acute psychological stress session decreased homotypic stressor. In contrast, sessions led release Acute injection(s) and transient lymphocytopenia. Further analysis showed repeated stress-induced lymphocytopenia is glucocorticoid-dependent. conclude can affect physiological parameters mice, potentially altering study results.