Long Non-coding RNA LINC00628 Interacts Epigenetically with the LAMA3 Promoter and Contributes to Lung Adenocarcinoma

摘要: Long non-coding RNAs (lncRNAs) have emerged as key regulators of cellular progress in lung adenocarcinoma. In this study, to identify cancer-related lncRNAs and genes, we screened for those that were differentially expressed adenocarcinoma, which revealed LINC00628 overexpression low expression laminin subunit alpha 3 (LAMA3). This was further validated the cancerous tissues from patients diagnosed with Thereafter, explored functional relevance LAMA3 adenocarcinoma by analyzing recruitment DNA methyltransferase (DNMT) processes cells following treatments induced or silencing 5-azacytidine (5-Aza, a DNMT inhibitor). The results showed decreased cell proliferation, migration, invasion well drug resistance vincristine (VCR). opposite demethylation induced 5-Aza treatment. Further research indicated recruited DNMT1, DNMT3A, DNMT3B promote methylation promoter, thereby decreasing its expression. Moreover, an in vivo experiment performed nude mice assess tumor growth ability human cells. It observed treatment inhibited human lung reduced their VCR. Altogether, our provide evidence mechanism exerts inhibitory role modulating LAMA3, indicative novel molecular target showing