Microbial community structure in the gut of the New Zealand insect Auckland tree weta (Hemideina thoracica)

作者: David W. Waite , Melissa Dsouza , Kristi Biswas , Darren F. Ward , Peter Deines

DOI: 10.1007/S00203-015-1094-3

关键词:

摘要: The endemic New Zealand weta is an enigmatic insect. Although the insect well known by its distinctive name, considerable size, and morphology, many basic aspects of biology remain unknown. Here, we employed cultivation-independent enumeration techniques rRNA gene sequencing to investigate gut microbiota Auckland tree (Hemideina thoracica). Fluorescence in situ hybridisation performed on different sections revealed a bacterial community fluctuating density, while gene-targeted amplicon pyrosequencing presence microbial containing high diversity, but apparent absence archaea. Bacteria were further studied using full-length 16S sequences, with statistical testing membership against publicly available termite- cockroach-derived revealing that similar cockroaches. These data represent first analysis provide initial insights into potential function these microorganisms.

参考文章(57)
Jorge W. Santo Domingo, Michael G. Kaufman, Michael J. Klug, James M. Tiedje, Characterization of the cricket hindgut microbiota with fluorescently labeled rRNA-targeted oligonucleotide probes Applied and Environmental Microbiology. ,vol. 64, pp. 752- 755 ,(1998) , 10.1128/AEM.64.2.752-755.1998
Michael W. Taylor, Peter J. Schupp, Ingela Dahllöf, Staffan Kjelleberg, Peter D. Steinberg, Host specificity in marine sponge-associated bacteria, and potential implications for marine microbial diversity. Environmental Microbiology. ,vol. 6, pp. 121- 130 ,(2004) , 10.1046/J.1462-2920.2003.00545.X
R I Amann, B J Binder, R J Olson, S W Chisholm, R Devereux, D A Stahl, Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Applied and Environmental Microbiology. ,vol. 56, pp. 1919- 1925 ,(1990) , 10.1128/AEM.56.6.1919-1925.1990
H J Gijzen, C A Broers, M Barughare, C K Stumm, Methanogenic bacteria as endosymbionts of the ciliate Nyctotherus ovalis in the cockroach hindgut. Applied and Environmental Microbiology. ,vol. 57, pp. 1630- 1634 ,(1991) , 10.1128/AEM.57.6.1630-1634.1991
Claire L. Thompson, Rahel Vier, Aram Mikaelyan, Tobias Wienemann, Andreas Brune, 'Candidatus Arthromitus' revised: segmented filamentous bacteria in arthropod guts are members of Lachnospiraceae. Environmental Microbiology. ,vol. 14, pp. 1454- 1465 ,(2012) , 10.1111/J.1462-2920.2012.02731.X
Priscilla M. Wehi, Brendan J. Hicks, Isotopic fractionation in a large herbivorous insect, the Auckland tree weta. Journal of Insect Physiology. ,vol. 56, pp. 1877- 1882 ,(2010) , 10.1016/J.JINSPHYS.2010.08.005
Mitsunori Tokura, Moriya Ohkuma, Toshiaki Kudo, Molecular phylogeny of methanogens associated with flagellated protists in the gut and with the gut epithelium of termites FEMS Microbiology Ecology. ,vol. 33, pp. 233- 240 ,(2000) , 10.1111/J.1574-6941.2000.TB00745.X
María José Gosalbes, Ana Durbán, Miguel Pignatelli, Juan José Abellan, Nuria Jiménez-Hernández, Ana Elena Pérez-Cobas, Amparo Latorre, Andrés Moya, Metatranscriptomic Approach to Analyze the Functional Human Gut Microbiota PLoS ONE. ,vol. 6, pp. e17447- ,(2011) , 10.1371/JOURNAL.PONE.0017447