A critical evaluation of sampling methods used for assessing microorganisms on surfaces

摘要: Abstract Methods used to evaluate the effectiveness of cleaning and disinfection regimes, or putative antimicrobial surfaces, rely on an estimate amount viable microbial cells remaining a surface after treatment. In essence, are applied surface, number specified time/treatment is assessed by variety methods. This paper provides critical commentary these The most common method relies removal from swabbing agitation, plating onto culture media, counting colonies obtained. However, should always be subsequently examined for residual cells: low numbers deemed indicative effective (i.e. few surface), but they could also indicate that have not been removed surface. Swabbing efficiency can affected moisture at presence organic material, topography compounds. It important aware limitations given assessing microorganisms as well intended property agent