The retina as a novel in vivo model for studying the role of molecules of the Bcl-2 family in relation to MPTP neurotoxicity.

摘要: To determine the roles of different members family B cell lymphoma protooncogene (Bcl-2) in relation to neurotoxin-induced neuronal degeneration, pattern expression a number molecules Bcl-2 was studied immunocytochemically retinas C57BL/6J mice after intraperitoneal (IP) injection 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Three days 12 weeks MPTP treatment, detectable reduction tyrosine hydroxylase immunoreactivity amacrine cells observed, with an increase Muller glial cells, and de novo Bad Bax retinal ganglion optic nerve fibers plexiform layers. In contrast, slight decrease Bcl-xL whereas Bcl-xS/L increased slightly MPTP-treated compared that controls. animals received injection, immunostaining GFAP, glutamine synthetase, Mac-1 (CD11b) astrocytes, microglia invariably indicating activation or dysfunction cells. These findings are consistent current view is important mediating cytotoxic effect variety neurotoxic molecules, including MPTP, may have as far degeneration neuroprotection concerned.