Imidazolium Salt (DBZIM) Reduces Gliosis in Mice Treated with Neurotoxicant 2 -CH3-MPTP

摘要: We have recently identified a class of imidazolium salts (IMSs) with antioxidative property and can function as scavengers for radical oxygen species (ROS) [18]. Here, we investigate one the IMSs, 1,3-bisbenzylimidazolium bromide (DBZIM), its possible role in attenuating neurotoxicity gliosis retina brain induced by Parkinsonian neurtoxicant, methyl-4(2 � methylphenyl)-1,2,3,6-tetrahydropyridine (2 -CH3-MPTP), which is free generating agent. In this study, employ molecular retinal imaging method, developed transgenic mouse model expressing green fluorescent protein (GFP) under control glial fibrillary acidic (GFAP) promoter [14], to assess efficacy DBZIM, since currently no vitro system sufficient complexity available accurately assessing compound’s efficacy. The longitudinal results showed DBZIM effectively suppress neurotoxicant-induced gliosis. Immunohistochemistry performed on postmodern confirmed that also reduced striatal gliosis, concomitantly attenuated loss dopaminergic neurons substantia nigra pars compacta (SNpc). These findings suggest could be useful small compound studying neuroprotection brain.