Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates.

摘要: The aim of this study was to reinvestigate the relationships and generic affiliations species Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, paraphrophilus segnis. nicotinamide phosphoribosyltransferase gene (nadV) conferring V factor-independent growth identified in aphrophilus. encodes a polypeptide 462 amino acids that shows 74.5 % acid sequence identity corresponding enzyme from actinomycetemcomitans. Ten isolates all carried nadV pseudogene. DNA aphrophilus able transform into NAD-independent phenotype. transformants full-length inserted former locus DNA-DNA relatedness between type strains 77 %. We conclude division two is not justified should be considered later heterotypic synonym Forty segnis were investigated by multilocus analysis. 40 form monophyletic group clearly separate other evolutionary lineages family Pasteurellaceae. propose transfer new genus Aggregatibacter gen. nov. as actinomycetemcomitans comb. (the species; strain ATCC 33384(T)=CCUG 13227(T)=CIP 52.106(T)=DSM 8324(T)=NCTC 9710(T)), (type 33389(T)=CCUG 3715(T)=CIP 70.73(T)=NCTC 5906(T)) HK316(T)=ATCC 33393(T)=CCUG 10787(T)=CCUG 12838(T)=CIP 103292(T)=NCTC 10977(T)). are independent X factor variably dependent on for vitro.