Properties of filament-bound myosin light chain kinase.

摘要: Myosin light chain kinase binds to actin-containing filaments from cells with a greater affinity than F-actin. However, it is not known if this binding in regulated by Ca2+/calmodulin as Therefore, the properties of stress fibers were examined smooth muscle-derived A7r5 cells. Full-length myosin or truncation mutant lacking residues 2–142 was expressed chimeras containing green fluorescent protein at C terminus. In intact cells, full-length bound fibers, whereas truncated showed diffuse fluorescence cytoplasm. After permeabilization saponin, disappeared, retained on fibers. Measurements intensities and recovery after photobleaching saponin-permeable that did dissociate these filaments. filament-bound sufficient for Ca2+-dependent phosphorylation regulatory contraction Thus, dissociation thin necessary thick We note distance between N terminus catalytic core span