Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho

摘要: The small GTP binding protein Rho is implicated in cytoskeletal responses to extracellular signals such as lysophosphatidic acid to form stress fibers and focal contacts. Here we have purified a Rho‐interacting protein with a molecular mass of approximately 164 kDa (p164) from bovine brain. This protein bound to GTPgammaS (a non‐hydrolyzable GTP analog). RhoA but not to GDP. RhoA or GTPgammaS. RhoA with a mutation in the effector domain (RhoAA37). p164 had a kinase activity which was specifically stimulated by GTPgammaS …