Knockdown of HMGB1 improves apoptosis and suppresses proliferation and invasion of glioma cells

摘要: Background: The purposes of this study were to explore the effects high mobility group protein box 1 (HMGB1) gene on growth, proliferation, apoptosis, invasion, and metastasis glioma cells, with an attempt provide potential therapeutic targets for treatment glioma. Methods: expressions HMGB1 in cells (U251, U-87MG LN-18) one control cell line (SVG p12) detected by real time PCR Western blotting, respectively. Then, biological behaviors detected: expression human lines U251 suppressed using RNAi technique, then influences viability, cycle, invasion abilities analyzed a Transwell chamber. Also, cyclin D1, Bax, Bcl-2, MMP 9 detected. Results: As shown real-time significantly increased U-87MG, comparison p12); vitality, proliferation invasive capabilities siRNA-transfected lower than those blank negative (NC) siRNA (P<0.05) but showed no significant difference between NC group. percentage apoptotic was higher similar latter two groups. had levels Cyclin MMP- Bax (P<0.05); profiles change both Conclusions: may promote migration suppress its apoptosis. Inhibition can their Our observations provided new target intervention