HMGB1 knockdown effectively inhibits the progression of rectal cancer by suppressing HMGB1 expression and promoting apoptosis of rectal cancer cells

摘要: Rectal cancer is a malignant gastrointestinal tumor, which associated with high morbidity and mortality. High‑mobility group protein 1 (HMGB1) widely present in the nucleus of eukaryotic cells, highly conserved between humans rodents. Recently, HMGB1 has been reported to be involved progression metastasis human cancer; however, its role development rectal remains unclear. The study detected expression levels pathological specimens from patients clinically identified using immunohistochemistry western blotting. results demonstrated that was expressed samples cancer. positive rate tissues 96.08% (49/51), significantly higher compared 3.92% (2/51) normal tissues. In addition, blotting indicated distributed located not only nucleus, but also cytoplasm colorectal cells. HMGB1‑specific short hairpin (sh)RNA used silence endogenous A functional assay knockdown inhibited proliferation SW620 Colo320 Furthermore, revealed contributed activation caspase‑3 substrate poly (ADP‑ribose) polymerase. B‑cell lymphoma 2 (Bcl‑2) Bcl‑2‑associated X (Bax) were by As expected, decreased Bcl‑2 increased Bax shRNA‑transfected Bax/Bcl‑2 ratio These data may act as an oncogene cancer, inhibit cells promote apoptosis tumor Further research regarding mechanisms underlying effects on provide novel targets for treatment theoretical reference clinical treatment.