Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media.

作者: Jonathan R. Brody , Eric S. Calhoun , Eike Gallmeier , Talisa D. Creavalle , Scott E. Kern

DOI: 10.2144/04374ST04

关键词:

摘要: Current DNA electrophoretic solutions employ high ionic concentrations and require long run times. Here we demonstrate that low molecular weight doublestranded DNA, single-stranded (ssDNA), RNA can be separated rapidly in agarosebased low-molarity conductive media. Separation of small fragments was optimized by substituting 1-mM alkali metals or a nonbiological amine distributed voltage with minute current. These ultra-dilute separate at least 15-fold faster. Low-molarity media 5–10 mM adequately larger as well. novel reduce the Joule heating system allow for easy-to-use, ultra-fast separation fragments.

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