Characterization and complete genome sequence analysis of two Myoviral bacteriophages infecting clinical carbapenem-resistant Acinetobacter baumannii isolates.
作者： J. Jeon , R. D'Souza , N. Pinto , C.‐M. Ryu , J. Park
DOI: 10.1111/JAM.13134
关键词:
摘要: AIMS The aim of this study was to characterize phenotypical properties, analyse whole genomes novel Acinetobacter baumannii phages infecting carbapenem-resistant Ac. baumannii (CRAB) and evaluate their potential as antimicrobial alternatives control in clinical settings. METHODS AND RESULTS phages, Βϕ-R1215 Βϕ-R2315, were isolated from sewage samples. These characterized by transmission electron microscopy, host spectrum, the thermal/pH stability test, bacterial lysis assay genome analysis. Both lysed 21 45 CRAB hosts, showed high at various pH (pH 4-10) temperature (25-60°C), strongly active against bacteria in vitro. Βϕ-R2315 are linear double-strands DNA with 44·866 44·846 bp respectively. two revealed similarity level, but organization direction open reading frames different. CONCLUSIONS lytic lysing strains which respiratory samples patients. SIGNIFICANCE IMPACT OF THE STUDY In vitro silico data that these have healthcare
elsevier.com
sci-hub.se 参考文章(29)
Andrew M. Kropinski, Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson, Enumeration of bacteriophages by double agar overlay plaque assay. Methods of Molecular Biology. ,vol. 501, pp. 69- 76 ,(2009) , 10.1007/978-1-60327-164-6_7
Paul Hyman, Stephen T. Abedon, Practical methods for determining phage growth parameters. Methods of Molecular Biology. ,vol. 501, pp. 175- 202 ,(2009) , 10.1007/978-1-60327-164-6_18
Emilie Saussereau, Laurent Debarbieux, Bacteriophages in the experimental treatment of Pseudomonas aeruginosa infections in mice. Advances in Virus Research. ,vol. 83, pp. 123- 141 ,(2012) , 10.1016/B978-0-12-394438-2.00004-9
P. Nordmann, L. Poirel, Emerging carbapenemases in Gram-negative aerobes Clinical Microbiology and Infection. ,vol. 8, pp. 321- 331 ,(2002) , 10.1046/J.1469-0691.2002.00401.X
Xavier Wittebole, Sophie De Roock, Steven M Opal, A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens Virulence. ,vol. 5, pp. 226- 235 ,(2014) , 10.4161/VIRU.25991
Rebekah A Frampton, Corinda Taylor, Angela V Holguín Moreno, Sandra B Visnovsky, Nicola K Petty, Andrew R Pitman, Peter C Fineran, None, Identification of bacteriophages for biocontrol of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Applied and Environmental Microbiology. ,vol. 80, pp. 2216- 2228 ,(2014) , 10.1128/AEM.00062-14
Hongjiang Yang, Li Liang, Shuxiang Lin, Shiru Jia, Isolation and Characterization of a Virulent Bacteriophage AB1 of Acinetobacter baumannii BMC Microbiology. ,vol. 10, pp. 131- 131 ,(2010) , 10.1186/1471-2180-10-131
Stephen T. Abedon, Sarah J. Kuhl, Bob G. Blasdel, Elizabeth Martin Kutter, Phage treatment of human infections. Bacteriophage. ,vol. 1, pp. 66- 85 ,(2011) , 10.4161/BACT.1.2.15845
Birendra R. Tiwari, Shukho Kim, Marzia Rahman, Jungmin Kim, Antibacterial efficacy of lytic Pseudomonas bacteriophage in normal and neutropenic mice models Journal of Microbiology. ,vol. 49, pp. 994- 999 ,(2011) , 10.1007/S12275-011-1512-4
Lenie Dijkshoorn, Alexandr Nemec, Harald Seifert, An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii Nature Reviews Microbiology. ,vol. 5, pp. 939- 951 ,(2007) , 10.1038/NRMICRO1789