作者: Pei-xiang Xing , Ian F. C. McKenzie
DOI: 10.1007/978-1-4615-3740-3_5
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摘要: Until recently most monoclonal antibodies to breast cancer were made by injecting whole tumours, crude tumour extracts or human milk fat globule membrane (HMFG) and selecting on the basis of a preferential reaction with lesser reactions normal tissues (1–5). A number so produced in have been useful development serum tests, imaging for therapy (6–10). Nonetheless, approach was crude, although reagents produced. By analysis many different around world, it appears that cancer, more than 90% react mammary mucins (11). Further, these could either carbohydrate determinants mucin (12), deglycosylated material, protein core (13); some appear require presence both (14,15,16). major breakthrough production came isolation cDNA clones coding part (17,18), later sequence obtained from laboratories (19,20). One feature this work small sequences λgtll expression libraries using - an unusual finding until realised able peptides inserts (see below). As discussed extensively elsewhere volume, remarkable VNTR (variable tandem repeats), where 60 bp repeat repeated 40–80 times (19, 20). It is anti-protein react. The amino acid N-terminal C-terminal now known studies are being performed determine whether immunogenic; however, current information involves 20 acids (encoded VNTR) will be herein.