Live-cell imaging and quantitative analysis of embryonic epithelial cells in Xenopus laevis.

摘要: Embryonic epithelial cells serve as an ideal model to study morphogenesis where multi-cellular tissues undergo changes in their geometry, such cell surface area and height, mitosis migrate. Furthermore, can also regulate morphogenetic movements adjacent tissues1. A traditional method involve chemical fixation histological methods determine morphology or localization of particular proteins interest. These approaches continue be useful provide "snapshots" shapes tissue architecture, however, much remains understood about how acquire specific shapes, various move localize positions, what paths follow toward final differentiated fate. High resolution live imaging complements allows more direct investigation into the dynamic cellular processes involved formation, maintenance, multicellular sheets. Here we demonstrate experimental from isolation animal cap Xenopus laevis embryos confocal simple measurement that together augment molecular studies morphogenesis.