Transgenic Xenopus laevis Line for In Vivo Labeling of Nephrons within the Kidney

摘要: Xenopus laevis embryos are an established model for studying kidney development. The nephron structure and genetic pathways that regulate nephrogenesis conserved between humans, allowing the study of human disease-causing genes. also amenable to large-scale screening, but studies disease-related genes have been impeded because assessment development has largely limited examining fixed embryos. To overcome this problem, we generated a transgenic line labels kidney. We characterize cdh17:eGFP line, showing green fluorescent protein (GFP) expression in pronephric mesonephric kidneys colocalization with known markers. demonstrate feasibility live imaging embryonic use as marker secretion assays. Additionally, develop new methodology isolate identify cells primary culture. morpholino knockdown essential establish GFP enables observation phenotypes, previously only described Taken together, will enable cell culture It provide simple means high-throughput screening putative