Address/capture tags for flow-cytometery based minisequencing

摘要: A method for generating address/capture tags use in a sensitive and rapid flow-cytometry based assay the multiplexed analysis of SNPs on polymerase-mediated primer extension using microspheres as solid supports is described. Single-nucleotide polymorphisms (SNPs) are most abundant type human genetic variation. These variable sites present at high density genome, making them powerful tools mapping diagnosing disease-related alleles. Subnanomolar concentrations sample small volumes (10 ml) can be analyzed rates greater than one per minute, without wash step. Genomic multiplexing microsphere arrays, enables simultaneous dozens, potentially hundreds sample. The has been tested by genotyping Glu69 variant from HLA DPB1 locus, SNP associated with chronic beryllium disease, well DPA1