Studying biological membranes with extended range high-speed atomic force microscopy

摘要: High—speed atomic force microscopy has proven to be a valuable tool for the study of biomolecular systems at nanoscale. Expanding its application larger biological specimens such as membranes or cells has, however, difficult, often requiring fundamental changes in AFM instrument. Here we show way utilize conventional instrumentation with minor alterations perform high-speed imaging large scan range. Using two—actuator design adapted control systems, 130 × 130 × 5 μm scanner nearly 100 kHz open—loop small-signal Z—bandwidth is implemented. This allows biologically relevant samples well measurements nanomechanical surface properties. We demonstrate system performance by real-time effect charged polymer nanoparticles on integrity lipid high speeds and peak tapping 32 kHz rate.