Apoptotic markers can be used to forecast the freezeability of stallion spermatozoa.

摘要: In an attempt to identify valuable markers for potential freezeability of the equine spermatozoa, three ejaculates were collected from five Andalusian stallions and frozen using a standard protocol. Before freezing, apoptotic cell studied by flow cytometry (early changes in sperm membranes, mitochondrial membrane caspase activity). Post-thaw, spermatozoa again evaluated these parameters. Sperm kinematics CASA also before after freezing thawing. Receiving operating system curves used evaluate relative value herein studied, as forecast freezeability. From all parameters outcome JC-1 (as proportion showing simultaneously orange green fluorescence) had highest diagnostic power. For potentially bad freezers (less than 25% intact post-thaw), significant area under ROC-curve was 0.985, with 100% sensitivity 99.8% specificity cut off 55.7.