Purification, Crystallisation and Preliminary X‐Ray Studies on Avian Pancreatic Polypeptide
作者： S. P. WOOD , J. E. PITTS , T. L. BLUNDELL , I. J. TICKLE , J. A. JENKINS
DOI: 10.1111/J.1432-1033.1977.TB11720.X
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摘要: A pancreatic polypeptide with some hormonal properties has been purified from chicken and turkey pancreas using acid-ethanol extraction, gel filtration anion-exchange chromatography. The material crystallised. crystals are monoclinic space group C2. Preliminary isomorphous replacement experiments have so far provided a single-site derivative Hg(N03)Z. low-resolution electron density map phased this anomalous scattering considered together Patterson function calculations suggest that the molecules partly helical arranged as compact dimer about crystallographic two-fold axis. structure association of these compared those insulin glucagon, protein hormones respectively, which studied in great detail. During studies on isolation insulin, Kimmel co-workers [l] side fraction. This chemically characterised data is accumulating in- dicates it third hormone [2]. Homologous polypeptides various mammalian sources sequenced [3]. avian comprises single chain 36 amino acids
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