Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA.

摘要: We have recently described the isolation of a novel protein, MIA, which is secreted from malignant melanoma cells and elicits growth inhibition on in vitro (Blesch, A., Bosserhoff, A. K., Apfel, R., Behl, C., Hessdorfer, B., Schmitt, Jachimczak, P., Lottspeich, F., Schlingensiepen, H., Buettner, Bogdahn, U. (1994) Cancer Res. 54, 5695-5701). Here, we report structure human MIA gene locus, describe its expression pattern melanocytic tumors vivo, provide an initial characterization promoter. The encoded by four exons, mRNA initiation site was identified 70 base pairs upstream translation start codon. vivo correlated with progressive malignancy lesions inducible other phorbol esters. To investigate mechanisms mediating this melanoma-associated pattern, analyzed promoter activity 1.3-kilobase genomic sequences located 5'-upstream gene. conferred high levels activation specifically murine cells, further enhanced treatment Site-directed mutation NF-kB within did reduce basal but not change significantly enhancement