Dexamethasone-induced increase in platelet-derived growth factor (B) mRNA in human alveolar macrophages and myelomonocytic HL60 macrophage-like cells.

摘要: Platelet-derived growth factor (B) (PDGF(B)) from alveolar macrophages is thought to play a central role in orchestrating the fibrotic response. Because corticosteroids are widely used treatment of patients with lung fibrosis, we asked whether modulated PDGF(B) gene activation macrophages. mRNA obtained smokers was increased after culture presence dexamethasone (P < 0.05), interferon-γ (IFN-γ) or both combination 0.05). Dexamethasone did not alter abundance encoding transforming factor-β (TGF-β), but decrease early response 2 (EGR2). These initial experiments required large numbers cells and thus were performed on smokers. The results reproduced when healthy nonsmoking volunteers measured by reverse-transcriptase polymerase chain reaction (RT-PCR). There an increase macr...